
                                  lindna 



Function

   Draws linear maps of DNA constructs

Description

   lindna draws linear maps of DNA constructs. It uses the graphical
   shapes ticks, ranges, and blocks to represent genetic markers (e.g,
   genes and ESTs) and places them according to their position in a DNA
   fragment. The markers can be organized in different groups. It reads
   an input file containing the names and positions of the genetic
   markers, and data for controlling the appearance of the markers
   (drawing commands). The output is a plot with with the linear DNA map.

Usage

   Here is a sample session with lindna


% lindna -graph cps 
Draws linear maps of DNA constructs
Commands to the lindna drawing program file [inputfile]: data.linp
Draw a ruler [Y]: 
      Open : Open blocks
    Filled : Filled blocks
   Outline : Black border
Type of blocks [Filled]: 

Created lindna.ps

   Go to the input files for this example
   Go to the output files for this example

Command line arguments

   Standard (Mandatory) qualifiers:
  [-infile]            infile     [inputfile] Commands to the lindna drawing
                                  program file
   -[no]ruler          boolean    [Y] Draw a ruler
   -blocktype          menu       [Filled] Type of blocks (Values: Open (Open
                                  blocks); Filled (Filled blocks); Outline
                                  (Black border))
   -graphout           graph      [$EMBOSS_GRAPHICS value, or x11] Graph type
                                  (ps, hpgl, hp7470, hp7580, meta, cps, x11,
                                  tekt, tek, none, data, xterm, png, gif)

   Additional (Optional) qualifiers:
   -maxgroups          integer    [20] Maximum number of groups (Integer 1 or
                                  more)
   -maxlabels          integer    [10000] Maximum number of labels (Integer 1
                                  or more)
   -intersymbol        selection  [Straight] Type of junctions between blocks
   -intercolour        integer    [1] Colour of junctions between blocks
                                  (enter a colour number) (Integer from 0 to
                                  15)
   -interticks         boolean    [N] Horizontal junctions between ticks
   -gapsize            integer    [500] Interval between ticks in the ruler
                                  (Integer 0 or more)
   -ticklines          boolean    [N] Vertical lines at the ruler's ticks
   -textheight         float      [1.0] Height of text. Enter a number <1.0 or
                                  >1.0 to decrease or increase the size,
                                  respectively (Number 0.000 or more)
   -textlength         float      [1.0] Length of text. Enter a number <1.0 or
                                  >1.0 to decrease or increase the size,
                                  respectively (Number 0.000 or more)
   -margin             float      [1.0] Width of left margin. This is the
                                  region left to the groups where the names of
                                  the groups are displayed. Enter a number
                                  <1.0 or >1.0 to decrease or increase the
                                  size, respectively (Number 0.000 or more)
   -tickheight         float      [1.0] Height of ticks. Enter a number <1.0
                                  or >1.0 to decrease or increase the size,
                                  respectively (Number 0.000 or more)
   -blockheight        float      [1] Height of blocks. Enter a number <1.0 or
                                  >1.0 to decrease or increase the size,
                                  respectively (Number 0.000 or more)
   -rangeheight        float      [1.0] Height of range ends. Enter a number
                                  <1.0 or >1.0 to decrease or increase the
                                  size, respectively (Number 0.000 or more)
   -gapgroup           float      [1.0] Space between groups. Enter a number
                                  <1.0 or >1.0 to decrease or increase the
                                  size, respectively (Number 0.000 or more)
   -postext            float      [1.0] Space between text and ticks, blocks,
                                  and ranges. Enter a number <1.0 or >1.0 to
                                  decrease or increase the size, respectively
                                  (Number 0.000 or more)

   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-graphout" associated qualifiers
   -gprompt            boolean    Graph prompting
   -gdesc              string     Graph description
   -gtitle             string     Graph title
   -gsubtitle          string     Graph subtitle
   -gxtitle            string     Graph x axis title
   -gytitle            string     Graph y axis title
   -goutfile           string     Output file for non interactive displays
   -gdirectory         string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages

Input file format

  Input files for usage example

  File: data.linp

Start   1001
End     4270

group
exons
label
Block   1011    1362    3       H
ex1
endlabel
label
Tick    1610    8       H
EcoR1
endlabel
label
Block   1647    1815    1       H
endlabel
label
Tick    2459    8       H
BamH1
endlabel
label
Block   4139    4258    3       H
ex2
endlabel
endgroup

group
repeat-regions
label
Range   2541    2812    [       ]       5       V
Alu
endlabel
label
Range   3322    3497    >       <       5       V
MER13
endlabel

   Blank lines in the input file are ignored.

   The file is organized in several fields separated by one or more space
   or TAB characters.

   The first non-blank line gives the start position of the DNA fragment
   to be drawn after the keyword 'Start'. The end position is on the
   second line following the keyword 'End'.

   Then, one or more groups (up to a maximum of 20) of genetic markers
   are defined. Each group begins with a line consisting of the keyword
   'group' and ends with a line consisting of the keyword 'endgroup'.

   If desired, a group name can be given on the very next line. Spaces
   and tabulations are allowed but only the first 20 characters of that
   name (including spaces) will be displayed.

   Next, the individual markers (up to a maximum of 1000) of the group
   are described. Each marker begins with a line consisting of the
   keyword 'label' and ends with a line consisting of the keyword
   'endlabel'.

   Do not start a new marker (by using a 'label' line) before you have
   closed the previous marker (by using an 'endlabel' line). You must end
   a previous marker specification before starting a new one. i.e. do not
   overlap marker specifications in the input file.

   For each marker, the line following the 'label' line describes the
   marker. Three types of markers can be drawn: ticks, blocks, and
   ranges.

   For a tick, the descriptive line begins with the keyword 'Tick',
   followed by the position of the tick in the DNA fragment, and a number
   from 0 to 15 specifying the color in which the tick will be drawn (a
   list of available colors is given below). If desired, one or more
   names can be written above the tick. In this case an optional letter H
   or V should be added to the line. H or V indicate whether the text
   will be horizontal or vertical, respectively. If no letter is
   specified, H is assumed by default. A list of one or more names can be
   given on the next lines with one name per line.

   For a block, the descriptive line begins with the keyword 'Block',
   followed by the start and end positions of the block in the DNA
   fragment, and the number of the block's color. If desired, one or more
   names can be written above the block. Names are given on the following
   lines. An optional letter H or V can be added to the end of the
   descriptive line to specify the orientation of the name(s).

   For a range, the descriptive line begins with the keyword 'Range',
   followed by the start and end positions of the range in the DNA
   fragment. Next on the line are the types of boundaries that are to be
   drawn. The user can choose among '>', '<', '[', ']' and '|' for both
   start and end boundaries. If none of these types is wanted for a given
   boundary, then a point '.' must be entered. Next is the color number.
   If desired, one or more names can be written above the range. Names
   are given on the following lines. An optional letter H or V can be
   added to the end of the descriptive line to specify the orientation of
   the name(s).

   For any type of markers, names will be written in the same color as
   the marker. Also, try to avoid long names.

   The colors defined in the PLPLOT graphics library are:

   0 "BLACK", 1 "RED", 2 "YELLOW", 3 "GREEN", 4 "AQUAMARINE", 5 "PINK", 6
   "WHEAT", 7 "GREY", 8 "BROWN", 9 "BLUE", 10 "BLUEVIOLET", 11 "CYAN", 12
   "TURQUOISE", 13 "MAGENTA", 14 "SALMON", and 15 "WHITE".

   This format can also be used for the program "cirdna". 

Output file format

   Output is to the specified graphics device.

  Output files for usage example

  Graphics File: lindna.ps

   [lindna results]

Data files

   None.

Notes

   Whether to draw a rule and the type of block-fill style must be
   specified. There are many other (optional) qualifiers to control the
   appearance of the plot which will be prompted for if the program is
   run with -options on the command line and.

References

   None.

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with a status of 0.

Known bugs

   None.

See also

   Program name Description
   abiview Display the trace in an ABI sequencer file
   cirdna Draws circular maps of DNA constructs
   pepnet Draw a helical net for a protein sequence
   pepwheel Draw a helical wheel diagram for a protein sequence
   prettyplot Draw a sequence alignment with pretty formatting
   prettyseq Write a nucleotide sequence and its translation to file
   remap Display restriction enzyme binding sites in a nucleotide
   sequence
   seealso Finds programs with similar function to a specified program
   showalign Display a multiple sequence alignment in pretty format
   showdb Displays information on configured databases
   showfeat Display features of a sequence in pretty format
   showseq Displays sequences with features in pretty format
   sixpack Display a DNA sequence with 6-frame translation and ORFs
   textsearch Search the textual description of sequence(s)

Author(s)

   Nicolas Tourasse (nicolas.tourasse © biotek.uio.no)
   Biotechnology Center of Oslo

History

   Written (2000) - Nicolas Tourasse
   Updated (2002) - Nicolas Tourasse

Target users

   This program is intended to be used by everyone and everything, from
   naive users to embedded scripts.

Comments

   None
