
                                 jaspscan 



Function

   Scans DNA sequences for transcription factors

Description

   jaspscan scans one or more DNA sequences for transcription factor
   binding sites from the JASPAR database. Matches are searched for using
   fast sequence word-matching, optionally allowing mismatches. Because
   the binding sites are so small, there will be many spurious (false
   positive) matches.

   An output file is written with information on the matches, including
   sequence ID and accession number, the start and end positions of the
   match in an input sequence and the sequence of the region where a
   match has been found. Binding factor information, where available, is
   given at the end of the matches for each matching entry.

Usage

   Here is a sample session with jaspscan


% jaspscan -both 
Scans DNA sequences for transcription factors
Input nucleotide sequence(s): tembl:m23100
Jaspar matrix set
         C : Core
         F : Fam
         P : Phylofacts
         N : CNE
         O : POLII
         S : SPLICE
Matrix set [C]: 
Comma separated matrix list [all]: ma0079
Threshold percentage [80.0]: 
Output report [m23100.jaspscan]: 

   Go to the input files for this example
   Go to the output files for this example

Command line arguments

   Standard (Mandatory) qualifiers:
  [-sequence]          seqall     Nucleotide sequence(s) filename and optional
                                  format, or reference (input USA)
   -menu               menu       [C] Matrix set (Values: C (Core); F (Fam); P
                                  (Phylofacts); N (CNE); O (POLII); S
                                  (SPLICE))
   -matrices           string     [all] The name 'all' reads in all matrix
                                  files from the selected
                                  JASPAR matrix set. You can specify
                                  individual matrices by giving
                                  their names with commas between then, such
                                  as:
                                  'ma0001,ma0015'.
                                  The case of the names is not important. You
                                  can specify a file of
                                  matrix names to read in by giving the name
                                  of the file holding the
                                  matrix names with a '@' character in front
                                  of it, for example,
                                  '@matrix.list'.
                                  Blank lines and lines starting with a hash
                                  character or '!' are ignored and all other
                                  lines are concatenated together with a comma
                                  character ',' and then treated as the list
                                  of enzymes to search
                                  for.
                                  An example of a file of matrix names is:
                                  ! my matrices
                                  ma0001, ma0002
                                  ! other matrices
                                  ma0010
                                  ma0032
                                  ma0053 (Any string is accepted)
   -threshold          float      [80.0] If the matrix score is greater than
                                  or equal to this percentage
                                  then a hit will be reported (Any numeric
                                  value)
  [-outfile]           report     [*.jaspscan] Output report file name

   Additional (Optional) qualifiers:
   -exclude            string     The names of any matrices to exclude from
                                  the 'matrices'
                                  list. Matrices are specified in the same way
                                  as for the
                                  selection list. (Any string is accepted)
   -both               boolean    [N] If set then both the forward and reverse
                                  strands are searched

   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -sformat1           string     Input sequence format
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outfile" associated qualifiers
   -rformat2           string     Report format
   -rname2             string     Base file name
   -rextension2        string     File name extension
   -rdirectory2        string     Output directory
   -raccshow2          boolean    Show accession number in the report
   -rdesshow2          boolean    Show description in the report
   -rscoreshow2        boolean    Show the score in the report
   -rstrandshow2       boolean    Show the nucleotide strand in the report
   -rusashow2          boolean    Show the full USA in the report
   -rmaxall2           integer    Maximum total hits to report
   -rmaxseq2           integer    Maximum hits to report for one sequence

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages

Input file format

   jaspscan reads any normal sequence USAs.

  Input files for usage example

   'tembl:m23100' is a sequence entry in the example nucleic acid
   database 'tembl'

  Database entry: tembl:m23100

ID   M23100; SV 1; linear; genomic DNA; STD; HUM; 2085 BP.
XX
AC   M23100; M32822;
XX
DT   06-JUL-1989 (Rel. 20, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 10)
XX
DE   Human insulin receptor (INSR) gene, exon 1, clones lambda-hINSR-(1-13).
XX
KW   Alu repeat; insulin receptor.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia
;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-1933
RX   PUBMED; 2911561.
RA   Seino S., Seino M., Nishi S., Bell G.I.;
RT   "Structure of the human insulin receptor gene and characterization of its
RT   promoter";
RL   Proc. Natl. Acad. Sci. U.S.A. 86(1):114-118(1989).
XX
RN   [2]
RP   1-2085
RX   PUBMED; 2210055.
RA   Seino S., Seino M., Bell G.I.;
RT   "Human insulin-receptor gene. Partial sequence and amplification of exons
RT   by polymerase chain reaction";
RL   Diabetes 39(1):123-128(1990).
XX
DR   EMBL-JOIN; M32972.
DR   TRANSFAC; R03068; HS$INSR_01.
DR   UniProtKB/Swiss-Prot; P06213; INSR_HUMAN.
XX
CC   Draft entry and computer-readable sequence for [1] kindly submitted
CC   by G.Bell, 14-MAR-1990.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2085
FT                   /organism="Homo sapiens"
FT                   /map="19p13.3-p13.2"
FT                   /mol_type="genomic DNA"
FT                   /dev_stage="foetus"
FT                   /tissue_type="liver"
FT                   /db_xref="taxon:9606"
FT   repeat_region   <1..76
FT                   /rpt_family="Alu"


  [Part of this file has been deleted for brevity]

FT                   /number=1
FT                   /note="alternative exon 1; G00-119-352"
FT   exon            1548..1923
FT                   /gene="INSR"
FT                   /number=1
FT                   /note="alternative exon 1; G00-119-352"
FT   sig_peptide     1824..1904
FT                   /gene="INSR"
FT                   /note="G00-119-352"
FT   intron          1924..>2085
FT                   /gene="INSR"
FT                   /number=1
FT                   /note="G00-119-352"
XX
SQ   Sequence 2085 BP; 417 A; 631 C; 702 G; 335 T; 0 other;
     agatctggcc attgcactcc agcctgggca acagagaaaa actccatcta aaaaaaaaaa        6
0
     aaaaaaaaaa aaaaaacaga gagagagaga gagagagaga gaaggaaacg gaactggggg       12
0
     gaggatttgc aaaaatatgg ttagggatgg cacttcagag atgaagccat cctggagtgt       18
0
     tacgggcaag ggaaatgctg gggcaaagcc ccagaggcag gaataggttt ggcctgttgc       24
0
     atgaacagtg ggtccagctc ctagcaaact gtttattgaa tgaaagaaga atgaatgcct       30
0
     tgggtctagg gttgtgctgg gcgctttctt aagttttctt tcccgggtac ctccccagaa       36
0
     ctggcatgca ggtattatta aacccattac acaagtgaaa ctggcccaga gacagaaaag       42
0
     tccctggtcc aagaccacac aggagtgagg ggtggaggaa ccctcctccc attgagttct       48
0
     ggctttccta tactgaaagc cccttcctct cctgcagtaa ggtaggtgga accgctgtcc       54
0
     cgccttgttg gtgaatgtcg ttgctagact tcagacacat acaggctggt ctgctgaaaa       60
0
     tcagagatgt ccacctgcgc cctattcgag gtctccggcg tcttctttgg cgtcgtcttt       66
0
     gccctttcag aagcgtctgc acatttttcc aggtgtcatt tctccaactt gaacacaggg       72
0
     agcgcactgg gcacgcgggc acgtggctgt ccccaggggc ctggcttggg tctcgcccct       78
0
     gggccggggc gcacgcgcgg gcgggacatc tgggggcgcc cacgcgctct gggacgagtg       84
0
     tcgctggcca ggcccggact gaggaaaggc gagtgagaca ctactcgcct ggggtgcaaa       90
0
     atttaaggga gtgaaaaaaa aaaaaaaaga aagaaaccaa aaccacctcg agtcaccaaa       96
0
     ataaacattt taatgcagta ttttttaaaa aatcaacagg aatcctccaa agcccactat      102
0
     gaacaaaata gcaaaatggt agagaaagga tctgtgccgc tgcgtcgggc ctgtggggcg      108
0
     cctccggggg tctgaaactg gaggagactc ggggctgtag ggcgcgcgga tctggggcgc      114
0
     gccctcggtc ccggcgcgcc cagggcctcc cgcgcggggc ccggcacagg gaggcgggga      120
0
     ggcgggcggg gcggggcggg accgggcggc acctccctcc cctgcaagct ttccctccct      126
0
     ctcctgggcc tctcccgggc gcagagtccc ttcctaggcc agatccgcgc cgccttttcc      132
0
     cgcggcccgc acggggccca gctgacgggc cgcgttgttt acgggccgga gcagccctct      138
0
     ctcccgccgc ccgcccgcca cccgccagcc caggtgcccg cccgccagtc agctagtccg      144
0
     tcggtccgcg cgtccctctg tcccggagcc cgcagatcgc gacccagagc gcgcggggcc      150
0
     gagagccgag agacagtccc gggcgcagcg cggagctccg ggccccgaga tcctgggacg      156
0
     gggcccgggc cgcagcggcc ggggggtcgg ggccaccacc gcaagggcct ccgctcagta      162
0
     tttgtagctg gcgaagccgc gcgcgccctt cccggggctg cctctgggcc ctccccggca      168
0
     ggggggctgc ggcccgcggg tcgcgggcgt ggaagagaag gacgcgcggc ccccagcgcc      174
0
     tcttgggtgg ccgcctcgga gcatgacccc cgcgggccag cgccgcgcgc tctgatccga      180
0
     ggagaccccg cgctcccgca gccatgggca ccgggggccg gcggggagcg gcggccgcgc      186
0
     cgctgctggt ggcggtggcc gcgctgctac tgggcgccgc gggccacctg taccccggag      192
0
     agggtgagtc tgggggcgcg ggcgtgggcg gggagcgccg cgatggggag aggaccccac      198
0
     ccaagccaaa atcgatcccc cgcttgtgga ctgagaaccc tccccagggg cggggggcgg      204
0
     tggccaggac ggtagctcct gcatcgcgta gggggagcgg gaagc                      208
5
//

Output file format

   jaspscan outputs a report format file.

  Output files for usage example

  File: m23100.jaspscan

########################################
# Program: jaspscan
# Rundate: Tue 15 Jul 2008 12:00:00
# Commandline: jaspscan
#    -both
#    -sequence tembl:m23100
#    -matrices ma0079
# Report_format: seqtable
# Report_file: m23100.jaspscan
########################################

#=======================================
#
# Sequence: M23100     from: 1   to: 2085
# HitCount: 18
#
# Database scanned: JASPAR_CORE  Threshold: 80.000
#
#=======================================

  Start     End  Strand Score_Percent ID     Name   Species      Class
  Supergroup Protein_Seq Experiment Source_PMID Info_Content Closest_Jaspar Clo
sest_Transfac MCS_Score Built_From Sequence
    117     126       +        82.143 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          gggggaggat
   1191    1200       +        89.286 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          gaggcgggga
   1203    1212       +        83.929 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          cgggcggggc
   1208    1217       +        89.286 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggcggggc
   1213    1222       +        80.357 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggcgggac
   1704    1713       +        82.143 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          cgggcgtgga
   1838    1847       +        82.143 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ccggcgggga
   1933    1942       +        82.143 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggcgcggg
   1945    1954       +        89.286 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          tgggcgggga
   2027    2036       +        89.286 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggcggggg
   2032    2041       +        87.500 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggggcggt
   2034    2043       +        82.143 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggcggtgg
   1252    1261       -        80.357 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          gagggaggga
   1304    1313       -        80.357 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          gcggcgcgga
   1805    1814       -        80.357 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          agcgcggggt
   1974    1983       -        89.286 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          tgggtggggt
   2016    2025       -        80.357 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          ggggagggtt
   2017    2026       -        87.500 MA0079 SP1    Homo sapiens ZN-FINGER, C2H
2 vertebrate P08047      SELEX      2192357            9.719 .              .
                      . .          tggggagggt

#---------------------------------------
#---------------------------------------

Data files

   EMBOSS data files are distributed with the application and stored in
   the standard EMBOSS data directory, which is defined by the EMBOSS
   environment variable EMBOSS_DATA.

   To see the available EMBOSS data files, run:

% embossdata -showall

   To fetch one of the data files (for example 'Exxx.dat') into your
   current directory for you to inspect or modify, run:

% embossdata -fetch -file Exxx.dat

   Users can provide their own data files in their own directories.
   Project specific files can be put in the current directory, or for
   tidier directory listings in a subdirectory called ".embossdata".
   Files for all EMBOSS runs can be put in the user's home directory, or
   again in a subdirectory called ".embossdata".

   The directories are searched in the following order:
     * . (your current directory)
     * .embossdata (under your current directory)
     * ~/ (your home directory)
     * ~/.embossdata

   The EMBOSS JASPAR transcription site files are stored in directory
   'data/JASPAR/*' under the EMBOSS installation directory.

   These files must first be set up using the program 'jaspextract'.
   Running 'jaspextract' may be the job of your system manager.

   The data files are stored in the JASPAR directory of the standard
   EMBOSS data directory. The subdirectory names are:
     * JASPAR_CORE Core files
     * JASPAR_FAM Family files
     * JASPAR_PHYLOFACT Phylogeny files

   The column information is described on the JASPAR home page
   http://jaspar.genereg.net/

Notes

   None.

References

    1. DNA binding sites: representation and discovery Bioinformatics.
       2000 Jan;16(1):16-23
    2. Applied bioinformatics for the identification of regulatory
       elements Nat Rev Genet. 2004 Apr;5(4):276-87

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with status 0.

Known bugs

   None.

See also

   Program name                         Description
   tfscan       Identify transcription factor binding sites in DNA sequences

Author(s)

   Alan Bleasby (ajb © ebi.ac.uk)
   European Bioinformatics Institute, Wellcome Trust Genome Campus,
   Hinxton, Cambridge CB10 1SD, UK

History

   Completed 23rd July 2007

Target users

   This program is intended to be used by everyone and everything, from
   naive users to embedded scripts.

Comments

   None
